ANAT 615. Techniques in Neuroscience and Cell Biology. 3 Hours.

Semester course; 4 lecture/lab hours. 3 credits. Recommended preparation: BIOC 503-504 or equivalent. Designed to provide in-depth coverage of techniques commonly used in neuroscience and cell biology. Topics include tissue processing for light and electron microscopy, immunocytochemistry, laser confocal microscopy, protein purification and analysis, molecular approaches to the study of the nervous system, genetic manipulation of protein expression, gene arrays, transgenic and knockout animal modes, and electrophysiological techniques including single and multi-unit extracellular recording, sharp intracellular recording and patch clamp recording. Consists of one two-hour meeting per week. Graded as Pass/Fail.